By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this ebook covers such issues as environmental overview of biotechnological approaches and microbial ameliorations of haloaromatic and haloaliphatic compounds.
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Extra info for Advances in Applied Microbiology, Vol. 3
Very few of the preparations stored at room temperature yielded a culture and those obtained from cultures stored at 4OC. developed slowly. By comparison, between 1 and 15%of the Vibrio he processed in the same manner remained viable after 9 to 12 months storage at room temperature. From the above discussion it seems that the differences among bacteria are as great or greater than the differences between bacteria and yeasts. Viruses are, as a whole, more sensitive to certain drying procedures, but they are more resistant to cold shock than are bacteria.
However, it is not easy to be certain that the protective colloid is adequate and hence the cell concentration may indeed be important. 08%of the cells remained viable after lyophilization. However his observations may have been obscured by the fact that as soon as the culture was diluted the cells began to grow, and, hence, Otten dried young cells which, as was shown in Section IV, B, are more sensitive to drying. Since Otten also showed that dead cells were protective it seems logical to expect a higher percentage of survivors from the more concentrated suspensions, particularly if no protective colloids are added, For instance, if one starts with a concentrated cell suspension of 1OI1 cells ' cells could die, and perhaps thus act as a protective colloid, up to 1OO yet one would observe 90%survival.
1951). 4. A portable manifold-type lyophilization apparatus. , Gardiner, New York. has not used any of the commercially available lyophilization equipment. However, the McLeod gage which is furnished as standard equipment by the VirTis Company is not satisfactory for lyophilization work for reasons discussed in Section 111, B. The American Sterilizer Company, Erie, Pennsylvania also manufactures lyophilization equipment designed primarily for tray drying and it can be used for pilot plant production.