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By Silvio Garattini

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Cruzi culture forms; data regarding bloodstream or tissue forms are not available so far. 3 . , 1969). , 1969). The presence of ergosterol may account for the activity of the antifungal polyene antibiotic amphotericin B against T . , 1964). Interaction between this antibiotic and cell membrane sterols, specially ergosterol, is supposed to increase the membrane permeability and cause the loss of low molecular components such as amino acids, ions, and soluble-fraction nucleotides (Ghosh and Chatterjee, 1961, 1962; Jaffe, 1968).

E. PHENANTHRIDINIUM COMPOUNDS Browning et al. (1946) were the first to demonstrate phenanthridinium derivatives containing carbethoxyamino groups to be active against T. cruzi in infected mice. Such compounds have been widely used for trypanosomiasis of cattle in Africa and in patients with Trypanosoma garnbiense (reviewed by Hawking, 1963). A large number of related compounds have been tested in T . cruzi infections and some 9phenylphenanthridinium salts with urethan substituents showed activity.

Parasitol. 63, 215-220. Brener, Z. (1971). Rev. Inst. Med. Trop. Sao Paulo 13, 302-306. Brener, 2. (1973). Annu. Rev. Microbiol. 27, 347-382. , and Chiari, E. (1963a). Rev. Inst. Med. Trop. Sao Paulo 5 , 128-132. , and Chiari, E. (196313). Rev. Inst. Med. Trop. Sao Paulo 5 , 220-224. , and Chiari, E. (1967). Rev. Inst. Med. Trop. Sao Paulo 9, 197-207. , and Chiari, E. (1971). Trans. Roy. Soc. Trop. Med. Hyg. 65, 629-636. , and Costa, C. A. G. (1974). Proc. Int. Cong. Parasitol. 3rd 3, 1292-1293.

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